previous arrow
next arrow
Slider

Quality of CHITOSAN products

. .

CHITOSAN product properties specification and control

  • Degree of Deacetylation / %DD

It is identify to be CHITIN - CHITOSAN, our CHITOSAN definition specific the degree of deacetylation  more than 80% of  by NMR (Nuclear Magnetic Resonance Spectroscopy) 

Degree of deacetylation using a NMR (Nuclear Magnetic Resonance Spectroscopy) Spectra of Chitosan samples (solid state) were recorded on Bruker AVANCE III HD(Ascend 400 WB)

  • MW / Molecular Weight 

By GPC (Gel Permeation Chromatography) method

Molecular weight of the sample was determined by GPC (Gel Permeation Chromatography) : The molecular weight of Chitosan samples (2mg/mL in acetate buffer) was estimated by GPC using pullulan(MW 5,800-788,000) as a standard. Analysis was performed on Ultrahydrogel linear column (7.8 ID x 300 mm, Waters, USA) connected in line with a Waters GPC apparatus. Samples were eluted with 0.5 M acetate buffer with a flow rate of 0.6 mL/min at 30 c and monitored by RI (refractive index) detector

  • Viscosity

By Cylinder Rotational Viscosity, Brookfield viscosmeter

Viscosity Chitosan at the concentration of 1 % was prepared by dissolving in 1% acetic acid. The viscosity of the Chitosan solution was determined using a Brookfield viscometer model LVDVD. To keep the temperature constant, Chitosan solution must be incubated at 25 c for 3 h before measuring

  • Soluble and insoluble 

% Insoluble by using  filtration method

  • ASH 

Ash content of the sample was determined by storing the sample in a muffle furnace at 600 c for 24 h or until the weight was constant. Ash content was calculated from the percentage of ash residue comparing to the initial weight of the moisture free sample
Remark: This method was modified from AOAC 938.08. 

  • Moisture

Moisture Content of the sample was determined by drying the sample at 105 c for 24 h or until the weight was constant. It was then calculated by percentage of weight loss comparing to the initial weight of the sample.
Remark: this method was modified from AOAC 934.01.

  • Particle size

Shaker : Lab Standard Sifters Shakers Test Sieve
Standard mesh (Tyler equivalent) (#50 mesh, #80 mesh and #230 mesh)

Heavy metals, microbiological specification and control

  • Heavy Metals

Arsenic : Test Method Based on AOAC(2005), specification NMT 0.5 ppm

Lead : Test Method LBCH-04408: Based on AOAC(2005), specification NMT 0.3 ppm

Cadmium : Test Method LBCH-04408: Based on AOAC(2005), specification NMT 0.5 ppm

Mercury :Test Method USEPA 7473/ ASTM 6722, specification NMT 0.1 ppm

  • Microbiological

Aerobic Plate Count : Test Method USFDA/CFSAN/BAM online (Chapter 3), specification NMT 100 cfu/g

Yeast & Mold : Test Method USFDA/CFSAN/BAM online (Chapter 18), specification NMT 100 cfu/g

E.coli & Coliform   : Test Method USFDA/CFSAN/BAM online (Chapter 4), specification None detected/1g

Staphylococcus aureus : Test Method USFDA/CFSAN/BAM online (Chapter 12), specification None detected/1g

Salmonella spp. :Test Method USFDA/CFSAN/BAM online (Chapter 5), specification None detected/25g

Listeria spp. : Test Method USFDA/CFSAN/BAM online (Chapter 10), specification None detected/25g

Pseudomonas : Test Method Based on ISO 13720, specification None detected/25g

  • Other

Melamine : Test Method USFDA , specification NMT 2.5 mg /kg

Benzo(a)pyrene : Test Method Based on journal of the Royal society of Chemistry, specification NMT 5.0μg/kg (wet weight)


Remark : NMT meaning is  "not more than"