CHITOSAN L112 BIOPOLYMER Research and Development

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How come our CHITOSAN L112 BIOPOLYMER from?

STUDY : OPTIMIZING CHITOSAN CHARACTERISTICS FOR EFFECTIVE USE IN HUMAN WEIGHT-LOSS REGIMENS

BULLET: ANTI-OBESE AND HYPOLIPIDEMIC EFFECTS OF CHITOSANS  IN RATS

Experimental designs : CHITOSANS of interest were CTS10, CTS28, CTS31, and CHITOSAN L112 from the CHITOSAN SPEC L112 CHARACTERISTICS STUDY : CHITOSANS OF INTEREST

What we have learned from that flake squidpen-derived chitosans functioned much better than powdered crab and shrimp chitosan in binding fats (corn oil, beef oil,olive oil and coconut oil). High-molecular-weight (HMW) squid pen chitosan had the highest fat-binding activity; however, the binding was not proportional to degree of deacetylation (DD). The chitosans preferred binding corn oil to olive oil, coconut oil and beef tallow. It is believed that the squidpen chitosans bound all fats better than crab and shrimp chitosans. Hydrophobic force (e.g. adsorption or van der Waals force) rather than electrical attraction is a fundamental factor in undigested (intact) lipid-binding performance of the chitosans. Hydrochloric acid pH 2.0 solution transformed colloidal chitosans into viscous chitosans, while the chitosans were precipitated in water and bicarbonate pH 8.5 solution. Swelling of the chitosans was unchanged in water and bicarbonate environment as in small intestine. Saying that, chitosans with small swelling index (SI) number will not enlarge intestinal luminal tract and make subjects uncomfortable. Formed fat-chitosan complex would be retained in the lumen shortly and excreted in feces eventually. It was found that CTS28  and CTS31  chitosans were very large and most effective in binding corn oil and beef tallow. Unfortunately, CTS10 chitosan showed the lowest fat-binding activity. Although they were administered per oral (po) and hydrolyzed partially in the stomach, residual chitosans matrices would retain the fat-binding property in small intestine. Nonetheless, we have not known what affinity of the chitosans for binding digested as well as pre-cooked fats is when compared to the intact (undigested) fats in our current study. Therefore, the chitosans are tested for their binding with pre-cooked fats in vitro. The selected chitosans are also examined for their weight-losing and hypolipidemic effects in prefatten as well as fattening rats. Change of plasma lipid profiles and body weight, liver function and kidney function are monitored in the treated rats.

OBJECTIVES OF THE STUDY

  • To investigate binding activity of selected chitosans with pre-cooked oils in vitro
  • To examine weight-losing activity of the chitosans in obese rats
  • To study effect of the chitosans on plasma lipid profiles in the treated rats
  • To monitor effect of the chitosans on liver and kidney functions